Journal of Young Pharmacists logo
Submit
Journal of Young PharmacistsACADEMIC PUBLISHING
Article

LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application

1,2★, 3, 4, 5★ Corresponding author

  1. 1Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University Kakinada, Kakinada–533 003, INDIA.
  2. 2Department of Pharmaceutical Analysis, Geethanjali College of pharmacy, Cheeryal–501 301, INDIA.
  3. 3Department of Chemistry, Yogi Vemana University, Kadapa–516 003, INDIA.
  4. 4Department of Pharmaceutical Chemistry, Surya School of Pharmacy, NH–45, GST Road, Vikravandi, Villpuram–605 652, INDIA.
  5. 5Department of Bioanalysis, PCR Laboratories, Ramanthapur, Hyderabad–500 013, INDIA.

CORRESPONDENCE

Anjaneyulu Narapusetti

Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru Technological University Kakinada, Kakinada–533 003, INDIA.

narapusetti@gmail.com

Volume 7, Issue 4s · pp. 438-445 · 2015 · DOI: 10.5530/jyp.2015.4s.5

ABSTRACT

Objective: The objective of this research was to develop a novel liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the determination of acetazolamide in human plasma.Methods: An analytical method based on LC-MS/MS (API-4000) has been developed and validated for the quantitative determination of acetazolamide in human plasma using acetazolamide d3 as Internal Standard (IS). After Solid phase extraction (SPE), analyte and the IS were chromatographed on a C18 columns using a isocratic mobile phase composed of 0.1% formic acid buffer and acetonitrile (30:70, v/v) pumped at a flow rate of 0.80 mL/min.Results: Precision and accuracy of the method was determined using five analytical batches in the concentration range of 50.3–12046 ng/mL. All the validation experiments were carried out as per the US FDA guidelines and results met the acceptance criteria.Conclusion: The proposed LC–MS/MS assay method is simple, rapid and sensitive for the determination of acetazolamide in human plasma. A chromatographic run time of 2.0 min, allow us to analyze more than 300 samples in a day.

KEYWORDS

Open in new tab

REFERENCES

As published

Showing references and in-text citations exactly as published.

    Cite this article

    SELECT FORMAT

    Narapusetti, A., Bethanabhatla, S. S., Sockalingam, A., & Pilli, N. R. (2015). LC–MS/MS assay for Acetazolamide, A Carbonic Anhydrase Inhibitor in Human Plasma and its Clinical Application. Journal of Young Pharmacists, 7(4s), 438-445. https://doi.org/10.5530/jyp.2015.4s.5

    Copyright and licence

    © 2015 Author(s)

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0