Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma
1★, 1, 1, 2★ Corresponding author
- 1Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KINGDOM OF SAUDI ARABIA.
- 2Department of Chemistry, College of Science, King Faisal University, Al-Ahsa,, KINGDOM OF SAUDI ARABIA.
CORRESPONDENCE
Mahesh Attimarad
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, KINGDOM OF SAUDI ARABIA.
Volume 6, Issue 4 · pp. 40-46 · 2014 · DOI: 10.5530/jyp.2014.4.7
ABSTRACT
Objective: The objective of the proposed method was to develop a simple, fast, sensitive, and validated high-performance liquid chromatography (HPLC) method for the simultaneous estimation of metformin and vildagliptin in tablets and human plasma.Materials and Methods: The chromatographic separation was accomplished with a fast monolithic column using a mixture of sodium dihydrogen phosphate and sodium dodecyl sulfate and acetonitrile at pH 4.5 as the mobile phase. In addition, newly developed method was validated as indicated by International Conference on Harmonization guidelines.Results:The method showed good correlation coeffi cients (r ≥ 0.997) in the range of 0.05-20 μg/mL and 0.1-40 μg/mL for metformin and vildagliptin, respectively. The accuracy and intraday and interday precision results were within the acceptable range for both analytes. The mean extraction recoveries of metformin and vildagliptin from human plasma were 97.51% and 97.18%, respectively.Conclusion: The simple, rapid, sensitive, robust and validated analytical method developed was used for simultaneous estimation of metformin and vildagliptin in formulation and in patient plasma.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Attimarad, M., Nagaraja, S. H., Aldhubaib, B. E., & Al-Najjar, A. (2014). Development of a rapid reversed phase-high performance liquid chromatography method for simultaneous determination of metformin and vildagliptin in formulation and human plasma. Journal of Young Pharmacists, 6(4), 40-46. https://doi.org/10.5530/jyp.2014.4.7
Copyright and licence
© 2014 Author(s)
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. CC BY-NC-SA 4.0